Histology slides for medical students

Collection of the pictures of important histology slides taken from a microscope . The images are arranged according to Systems and are extremely useful for medical students.


Cuboidal gland

Cube shaped, central nucleus.
eg. salivary, pancreatic duct, collecting tubules of kidney
histology slide of cuboidal gland

Elastic cartilage of epiglottis

1. Condrocytes are present in lacunae.
2. Matrix is made of elastic fibres predominantly.
3. Perichondrium is present.
histology slide of elastic cartilage

Brown Adipose Tissue

histology slide of brown adipose tissue

Thick skin

1. Lined by stratified squamous epithelium.
2. Presence of keratin layer.
3. Epidermal projections into dermis are epidermal papillae.
4. Dermal projections into epidermis are dermal papillae.

 histology slide of thick skin

Thin skin

1. Presence of hair follicles.
2. Lined by stratified squamous keratinized epithelium.
3. Presence of dermis and epidermis.
 histology slide of thin skin

Hyaline cartilage of bronchus

1. Presence of pericondrium.
2. Presence of cell nests.
3. Presence of condrocytes in the cell nests.
4. Matrix contains collagen fibers.
histology slide of hyaline cartilage of bronchus

Lymph Node

Presence of capsule and subcapsular sinus
Differentiated into cortex and medulla
Cortex has follicles: primary and secondary (pale staining germinal centre).
histology slide of lymph node


It has both lingual and laryngeal surface
Lingual surface is lined with stratified squamous non keratinizing epithelium while laryngeal surface is lined with pseudostratified ciliated columnar epithelium
Central elastic cartilage forms its framework
histology slide of epiglottis

Fibrous cartilage

histology slide of fibrous cartilage

Muscular Artery

Innermost tunica intima with endothelium and subendothelial connective tissue
Thick middle layer is tunica media with smooth muscles and elastic fibre in form of internal and external elastic lamina
Outermost tunica adventitia consists of both collagen and elastic fibers
histology slide of muscular artery

Non Keratinized epithelium

A stratified squamous epithelium consists of squamous (flattened) epithelial cells arranged in layers upon a basal membrane. Only one layer is in contact with the basement membrane; the other layers adhere to one another to maintain structural integrity.
eg oral mucosa, vagina,anal canal
histology slide of non keratinized epithelium

Skeletal muscle

1. Muscle fibers donot branch.
2. Nucleus is located in the periphery.
3. Presence of cross striations.
histology slide of skeletal muscle

Smooth muscle

1. Myocytes are spindle shaped.
2. Myocytes contain central nuclleus.

 histology slide of smooth muscle


Compact linear collagen fibers seen.
Few scattered, inactive fibroblasts with nuclei
Poor vascularity, tiny nerve endings and tendon stretch receptors see.
histology slide of tendon

Reticular fibers

histology slide of reticular fibers

Caridac muscle

1. Muscle fibers are branching.
2. Nucleus is centrally placed.
3. Presence of intercalated discs.
histology slide of cardiac muscle

Musculo skeletal System

Bone - TS

The osteocytes are arranged in concentric rings of bone matrix called lamellae (little plates), and their processes run in interconnecting canaliculi. The central Haversian canal, and horizontal canals (perforating/Volkmann's) canals contain blood vessels and nerves from the periosteum.
histology slide of transverse section of bone

Cancellous bone

Bones are much more porous with larger haversian canals and fewer lamellae.
Gives characteristic honeycomb appearance. Adaption to compressive forces.
Present near epiphyseal region of long bones, iliac crest.
histology slide of brown adipose tissue

Central Nervous System


1. Discrete aggregation of neurone cell bodies located outside CNS.
2. Nuuclei tend to be eccentrically located.
3. Satellite cells are smaller in number & irregularly placed due to numerous dendritic process.
histology slide of ganglion

Peripheral Nerve - LS

1. Cut section of axons are seen.
2. Axons lined by myelin sheath.
3. Nodes of Ranvier seen.
histology slide of peripheral nerve

Peripheral Nerve - TS

1. Cut section of nerve fiber bundles are seen.
2. Perineurium is seen.
histology slide of transverse section of peripheral nerve


 Located in post cranial fossa and covered superiorly by the tentorium cerebelli.
A. Outer grey matter:
i. Outer molecular layer which contains stellate and basket cells & their processes.
ii.Purkinje layer contains purkinje cells and golgi cells.
ii. Inner granular layer contains numerous granule cells.
B. Inner white matter: Deeply convulated folds or folia supported by a branching central medulla of white matter.
histology slide of cerebellum


histology slide of cerebrum

Spinal cord 

histology slide of spinal cord


Retina of eye has 8 layers. From outer to inner:
1. Pigment cell layer
2. Layers of rods and cones
3. External nuclear layer
4. External plexiform layer
5. Inner nuclear layer: has 3 types of neurons Bipolar neurons, horizontal neurons and amacrine cells
6. Internal plexiform layers
7. Layer of ganglion cells
8 Layers of optic nerve fibers
histology slide of eye

histology slide of filliform papillae

Tongue fungiform papillae

histology slide of fungiform papillae of tongue


histology slide of ileum


histology slide of jejunum


Hepatocytes extend as cord between central vein and portal triad in each corner
Bile canaliculi and hepatic sinusoids are present
Portal triad structure with bile duct,hepatic arteriole and hepatic venule are present.


histology slide of duodenum


Mucosa with stratified squamous epithelium.
Small seromucous gland in the submucosa
Muscularis propria differentiated into outer longitudinal and inner circular.
histology slide of esophagus

Cardio-esophageal junction

Abrupt transition from stratified squamous epithelium to glandular mucosa
Muscularis propria of esophagus is continuous with stomach
Esophageal cardiac glands are present in lamina propria.
histology slide of cardio-esophageal junction


Lymphoid masses within mucosa and submucosa often forming germinal center
Mucosal glands are much less closely packed than large intestine


Closely spaced straight tubular glands or crypts that extends deep to muscularis mucosa
Epithelium consists of goblet cells and columnar absorptive cells
Three longitudinal muscle layers called taenia coli are present.
histology slide of colon

Gall bladder

Mucosa is thrown into numerous folds with loose submucosa
Fibres of muscular layer are arranged in longitudinal, transverse and oblique orientation and don’t form distinct layers
Tall columnar cells with basal nuclei are seen.

histology slide of gall bladder


histology slide of GB


Lobulated organ with collagenous capsule which extends as septa
Exocrine component made by pancreatic acini
Endocrine component composed of islets of langerhans.
histology slide of pancreas

Pancreato-duodenal junction

Mucosal ridges become broader, more irregular.
Lymphatic nodules are seen between the stomach and duodenum
Mucous secreting stomach epithelium changes to intestinal epithelium(goblet cells with microvilli)
histology slide of pancreato-duodenal junction

Parotid gland

Gland(serous) is divided into numerous lobules with secretory unit
Presence of connective tissue septa extending from capsule with blood vessels, nerves seen
Intercalated duct also seen
Presence of lobules and connective tissue septa is common feature of all three types of gland
We need not differentiate, simply identify as salivary gland.
histology slide of parotid gland


histology slide of spleen

body of Stomach

histology slide of body of stomach

Sublingual gland

Mucous acini are predominant forms.
histology slide of sublingual gland

Submandibular gland

Mixed mucous and serous cells seen
Mucous cells(lightly stained) have serous demilunes(deeply stained)
 histology slide of submandibular gland

Cardio vascular system


Endothelium with subendothelial connective tissue
Elastic fibres (more) and muscles constitute the bulk of tunica media
Tunica adventitia with connective tissue and vasa vasorum.
histology slide of aorta


Tunica adventitia with vasa vasorum and longitudinal smooth muscle layers
Tunica media with thin layer of circular muscles
Endothelium with subendothelial connective tissue.
histology slide of venacava

Respiratory System


Incomplete C shaped cartilage present
Trachealis muscle is present posteriorly where cartilage is incomplete
Lined by pseudostratified ciliated columnar epithelium
histology slide of trachea


Flattened cartilage plates are present
Lined by respiratory epithelium with fewer goblet cells
Smooth muscles between lamina propria and submucosa which become prominent in distal bronchi
histology slide of bronchus


* In its histologic section, there are bronchi, bronchioles(terminal and respiratory) and alveolar sac and alveoli.
* Alveoli are small air filled pouches separated by alveolar septa.
* flattened pneumocytes and rich capillary network present.
histology slide of lung

Reproductive System

Vas deferens

It has inner and outer longitudinal muscle layer and intermediate circular layer(same like urinary bladder)
Lined by pseudostratified epithelium thrown into folds
Lamina propria beneath the epithelium with collagen
 histology slide of vas deferens


histology slide of epidiymis

Graffian Follicles

histology slide of graffian follicles


Composed of glands and stroma
Stroma made of supporting collagenous tissue with smooth muscle
Glands with convoluted pattern lined by tall columnar secretory cells with flat basal cells
Inspissated calcified concretions may accumulate forming corpora amylacea
histology slide of prostrate

Seminal vesicle

Highly irregular and recessed lumen giving a honeycomb appearance
Epithelial lining is of pseudostratified columnar with secretory cells and lipid droplets in cytoplasm giving it foamy appearance
Prominent muscular wall with arrangement into inner circular and outer longitudinal layer
histology slide of seminal vesicle


Dense fibrous capsule outside called tunica albuginea and has:
myofibroblasts, smooth muscles
Deepest layer of tunica albuginea called tunica vasculosa has blood and lymphatics
Inside testis:
There are lobules each with many seminiferous tubules lined by:
Germ cells in various stages of spermatogenesis and spermiogenesis collectively called spermatogenic series
Non germ cells called sertoli cells
In the interstitial cells, there are supporting leydig cells.
histology slide of testis


3 columns of erectile tissues
Two dorsal columns are called corpora cavernosa and one ventral column is called corposa spongiosum
Through corpora spongiosum, penile urethra passess
Erectile corposa are enclosed within and separated by fibrocollagenous septa F
Finally enclosed within the sheath of skin with blood vessels
histology slide of penis

Mammary gland in Lactating stage

Large no. of distended alveoli filled with secretions and vacuoles
Irregular branching of alveoli
Increased presence of adipose tissue
In mammary gland typical features: no need to distinguish lactating and resting
Adipose tissue with fibrous septa
Branching tubuloacinar gland
Glandular epithelium with epithelial and myoepithelial cells
Note : mammary gland could be confused with thyroid; generally mammary gland is more or less heterogenous while thyroid is homogenous.
histology slide of mammary gland in lactating stage

Mammary gland in resting stage

histology slide of mammary gland in resting stage

Uterus Secreting

In all, uterus has perimetrium,myometrium and endometrium
Endometrium has stratum basalis,stratum compactum and spongiosum
Proliferative: Straight tubular glands; Increased thickness of endometrium
Secretory: Coiled tubular glands; Increased thickness of endometrium.
 histology slide of uterus in secreting phase

Uterus proliferating

 histology slide of uterus in proliferating phase


Has two layers: germinal epithelium and stroma
Stroma has: cortex and medulla
Cortex: outer(tunica albuginea) and inner(follicles at various stages)
Medulla: has helicine arteries and hilus cells
histology slide of ovary


histology slide of oviduct


Cervical canal in cervix is lined with tall mucus secreting columnar epithelium
Branched tubular cervical glands also seen
Lamina propria with more fibrous tissue, blood vessels seen.
In the lower end of cervix, the os cervix bulges into lumen of vaginal canal and epithelium abruptly changes there from columnar to stratified squamous.
histology slide of cervix


Mucosa is irregular with folds
Lamina propria with dense irregular connective tissue and lymphatics
Predominantly oblique layer of muscles present in muscularis propria.
Adventitia with blood vessels and nerve fibres.

 histology slide of vagina

Renal System


Presence of transitional epithelium
Upper two third contains inner longitudinal and outer circular @COLI
(COLI in ureter (@since e.coli is common in lower urinary tract)
Lower one third contains additional outer longitudinal layer.
histology slide of ureter

Urinary bladder

It resembles lower one third of ureter:
The urinary bladder slide in our college is not of human( in human, preparation of complete circumference would not be possible)so slide in college have complete circumference. One way to distinguish ureter and urinary bladder is to compare magnification.
If we see complete lumen in 4 magnification, its urinary bladder
If we seen completer lumen in 10 magnification, its ureter. So look in focused lens also
histology slide of urinary bladder

Glomerulus of Kidney

histology slide of glomerulus of kidney


Adrenal cortex

Outer cortex and inner medulla
Prominent vein V in the center.
Cortex has 3 regions:
Zona glomerulosa: secretory cells in rounded clusters
Zona fasciculata: parallel cords of secretory cells
Zona reticularis: small closely packed cells in irregular cords
histology slide of adrenal cortex


histology slide of thymus


Consists of thyroid follicles and parafollicular cells(C cells)
Parafollicular cells are present in interstices between the follicles
Thyroid follicles has:
Cuboidal and flat lining epithelium (at inactive)
Columnar and tall lining epithelium( at active)
Homogenous colloid inside (stained pink)
 histology slide of thyroid

Pituitary gland

Two main population of cells:
Chromophils(strongly staining cytoplasm)
Chromophobes(weakly staining cytoplasm)
Chromophils are also two types:
Capillaries between the clumps of secretory cells.
histology slide of pituitary gland


histology guide, histology slides, histology book, histology report.

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